摘要 目的：建立高效液相色谱法测定沙拉沙星注射液中沙拉沙星的含量的方法。方法：采用Symmetry C18（250 mm×4.6 mm，5μm）色谱柱，以0.025mol/L磷酸溶液-乙腈(82∶18)为流动相（pH=3.0），流速1.0 ml/min，检测波长270nm。结果：沙拉沙星注射液在1-100μg/ml浓度范围内呈现良好的线性关系，相关系数R2为0.9994，平均回收率（n=6）为85.73%，RSD为4.64%。结论：该法准确、简便，适用于沙拉沙星注射液的含量测定。
关键词：沙拉沙星 注射液 含量测定 高效液相色谱法
Abstract objective:To establish an HPLC method for the determination of Sarafloxacin injection. Methods:The analysis was carried on Symmetry C18 column (250 mm×4.6 mm, 5μm). The mobile phase was 0.025mol/L phosphoric acid in acetonitrile(82:18)(adjust to pH3.0);the detecting wavelength was 270 nm and flow rate 1.0 ml/min. Results:The range of Sarafloxacin was 1.0−100.0 μg/ml with r2=0.9994,showing very good linear relationship.The mean recovery was 85.73% and the relative standard deviation was 4.64%.Conclusion:The method is simple, accurate and can be used for the determination of Sarafloxacin infection.
Key words:Sarafloxacin infection determination HPLC
提示：本站支持手机（IOS，Android）下载论文，如果手机下载不知道存哪或打不开，可以用电脑下载，不会重复扣费
