原子吸收法检测植物提取物中重金属的方法研究_食品质量与安全.doc

目的：本研究利用应用氢化物原子吸收法和石墨炉原子吸收法，通过检测器测定的吸光度来判定甘草酸单铵盐中的铅和砷的含量，通过加标回收的方法验证湿法消解的准确度和可行性。

　　　方法：本实验通过湿法消解的方法对样品进行处理，处理后进入氢化物检测器或石墨炉检测器进行检测。通过实验得出的吸光度来判定样品中铅和砷的含量。并通过对样品加入0.5倍和1.0倍的标准品来计算其加标回收率。

结果：（1）该甘草酸单铵盐中重金属砷的含量为：254.7ng/g。（2）加标回收的过程中回收率为：96.9%、106.1%，达到90%-110%。已验证该方法的可行性。（3）该甘草酸单铵盐中重金属铅的含量为：141.6ng/g。（4）加标回收的过程中回收率为：104.3%、106.6%,达到90%-110%。已验证该方法的可行性。

结论：运用原子吸收光谱的石墨炉原子吸收法和氢化物原子吸收法的方法是一种有效的、快速的检测方法，具有高灵敏度、用量少等优点，适合用于重金属检测中铅和砷的定量检测。

关键词：原子吸收光谱；氢化物检测器；石墨炉检测器；甘草酸单铵盐；重金属；砷；铅；湿法消解；加标回收率

Objective:In this study, the hydride generation atomic absorption method and graphite furnace atomic absorption spectrometry were applied to determine the content of lead and arsenic in Glycyrrhizic acid ammonium salt. The accuracy and feasibility of the wet digestion method was verified by the recovery of standard addition method.

Methods: The samples was treated by wet digestion method and then was detected by the hydride detector or graphite furnace detector. Absorbance was obtained to determine the content of lead and arsenic in the sample. The recovery rate was caculated by 0.5 times and 1.0 times added-standard. 

Results: (1) The As content of Glycyrrhizic acid ammonium salt was 254.7ng / g. (2) Recovery rate of added-standard was 96.9%, 106.1%, up to 90% -110%.The feasibility of this method was verified. (3) Pb content of the Glycyrrhizic acid ammonium salt was 141.6ng / g.(4) The recovery rate of spiked recovery process as follows: 104.3%, 106.6%, 90% -110%. The feasibility of this method was effective. 

Conclusion: The use of atomic absorption spectrometry graphite furnace atomic absorption spectrometry and hydride generation atomic absorption spectrometry method is an effective, rapid detection methods, with high sensitivity, less amount. suitable for the quantitative detection of lead and arsenic in the detection of heavy metals.

Key Words：Atomic absorption spectrometry; hydride detector; graphite furnace detector; Glycyrrhizic acid ammonium salt; heavy metals; As; Pb; the wet digestion; spiked recoveries