药食两用小蓟中组分A的分离纯化_生物技术.doc

摘要

　　　目的：小蓟是菊科蓟属植物，又名刺儿菜，是一种食疗兼备的传统中药。具有凉血、止血和祛瘀等功效。对小蓟石油醚萃取部位的组分A部分进行分离纯化与鉴定，为进一步开发利用小蓟提供科学依据。方法：应用各种分离纯化技术，包括正、反相常压色谱和制备型HPLC以及凝胶(Sephadex LH-20)等色谱方法，对其进行系统的分离纯化。并用1D-NMR波谱技术，对分离得到的化合物的化学结构进行分析鉴定。结果：从小蓟的石油醚萃取部位的组分A部分中分离得到1个化合物，并确定其结构为羽扇豆酮。

关键词：小蓟；分离纯化；羽扇豆酮

Abstract

　　　Objective: Cirsium setosum（Wild.）MB. belonging to Cephalanoplos Cirsium family, also known as Ci-Er-Cai, is a edible and traditional Chinese medicine. Cirsium setosum has the function of cooling blood, hemostasis, removing blood stasis and so on. This paper describes chemical investigations on the fraction B of petroleum ether extract of Cirsium setosum, to provide the scientific basis for further development and utilization. Methods: A variety of chromatographic techniques, including RP-pressure chromatography, HPLC and gel (Sephadex LH-20) chromatography method, were applied for isolation and purification. Its structure was identified by 1D NMR spectroscopic methods. Results: One compound was isolated from the fraction B of petroleum ether extract of Cirsium setosum, and elucidated as lupenone.

Key words: Cirsium setosum; isolation and purification; lupenone