富锌香菇菌丝体不同溶剂提取物的生物学活性研究_生物工程.doc

摘要:本文就富锌香菇菌丝体的不同溶剂提取物对氧自由基、羟自由基的抑制能力和T-SOD活力进行了测定。

　　　100μg/mL的锌对香菇菌丝体生长有促进的作用，富锌香菇提取物的生物活性比未富锌香菇提取物的生物活性高。通过微波消解-原子吸收法测定有机锌的含量。

　　　利用T-SOD试剂盒以及羟自由基试剂盒试剂进行总SOD活力，羟自由基抑制能力的测定。经过数据分析得，富锌香菇提取物的萃取时间越长，对氧自由基抑制率越高，对羟自由基抑制能力越强，总SOD活力越大。当萃取时间为30分钟时，富锌香菇水提取物对氧自由基抑制率最高为44.53％，且总SOD活力最大为434.57（U/ml）。当萃取时间为30分钟时，富锌香菇乙醇提取物对羟自由基抑制能力最高为392.79（U/ml）。

关键词：香菇；富锌；抗氧化；有机锌

Abstract:This paper researched the clearing ability of different extracts of zinc-rich mushroom mycelium on oxygen free radicals and hydroxyl radicals and on T-SOD activity.

　　　The 100μg/mL zinc promote the growth of shiitake fungus , the biological activity of than the non-zinc-rich mushroom extract is higher than  the biological activity of the no zinc-rich one. The determination of organic zinc content is by Microwave Digestion - Atomic Absorption Spectrometry .

    The determination of activity of SOD and the hydroxyl radical suppression ability is by T-SOD kit and hydroxyl radical kit reagents . After data analysis , the longer the extraction time of the zinc-rich mushroom extract, the higher the rate of O2-radical scavenging, the stronger hydroxyl radical scavenging and the greater the activity of SOD. When the extraction time of 30 minutes, the O2- radical inhibition rate and the total SOD activity of the zinc-rich mushroom water extract  is highest ,which is respectively up to 44.53% and 434.57 U / ml. When the extraction time of 30 minutes, the hydroxyl radical rejection capability of the zinc-rich mushrooms ethanol extract  is highest which is up to 392.79 (U / ml).

Key words  lentinus edodes  zinc-rich   antioxidation  Organic zinc