摘 要:目的：从转基因大豆中提取DNA并对大豆中转基因成分进行检测。方法：CTAB法、PCR扩增技术、设计并合成引物对转基因大豆的内源基因Lectin；外源基因CaMV35S 启动子、NOS终止子和Cp4 EPSPS基因进行检测。结果：转基因大豆中含有内源基因Lectin；外源基因CaMV35S 启动子、NOS终止子和Cp4 EPSPS基因。
关键词:转基因大豆；转基因成分定性PCR检测；Lectin基因；CaMV35S基因；Cp4 EPSPS基因
Abstract：Objective: To extract DNA from genetically modified soybean and to detect Genetically Modified Components in Soybean. Methods: DNA was extracted from soybean by CTAB method, the technique of PCR amplification, The primers for endogenous gene Lectin of genetically modified soybean, CaMV35S promoter gene , NOS terminator and Cp4 epsps gene were designed and synthesized. Results: There are endogenous gene Lectin , CaMV35S promoter gene , NOS terminator and Cp4 epsps gene in genetically modified soybean.
Key words：genetically modified soybean; qualitative PCR detection of genetically modified organisms; Lectin gene; CaMV35Sgene; Cp4 EPSPS gene.
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