富锗金针菇抗氧化、抗衰老能力研究.doc

摘 要：以富锗金针菇为研究材料，通过对富锗金针菇菌丝体清除超氧自由基能力、羟自由基能力以及小鼠血清中SOD和MDA含量的测定，对其抗氧化和抗衰老能力进行研究。

　　　实验结果表明：500μg/mL的锗对金针菇菌生长有促进的作用。70%乙醇醇析所得的空白金针菇多糖的得率较高，得率为5.064%，富锗金针菇多糖的得率比空白的金针菇多糖的得率高，为7.741%。通过对比表明，测定抗氧化能力时用水提取菌丝体比用乙醇要好，富锗组的SOD活性和抑制羟自由基能力分别为41.116 U/mL和34.674 U/mL，空白组的SOD活性和抑制羟自由基能力分别32.49 U/mL和29.006 U/mL。测小鼠血清可以看出富锗组的SOD含量和MDA含量分别为90.27 U/mL和9.381nmoL/mL，空白组的SOD含量和MDA含量分别为84.74 U/mL和12.201 nmoL/mL。由此可知富锗金针菇的抗氧化、抗衰老能力明显高于正常金针菇。

关键词：金针菇；微量元素；锗；抗氧化；抗衰老

Abstract：Study on Germanium- rich of Flammulina velutipes materials through on Germanium- rich of Flammulina velutipes mycelium in scavenging oxygen free radical and hydroxyl free radical and determination of SOD and MDA in serum of mice, a study on their antioxidant and anti-aging capacities.

　　　Experimental results show that the 500 μg/mL of Germanium on the growth-promoting effect of Flammulina velutipes. 70% ethanol of alcohol derived from analysis of blank Flammulina velutipes polysaccharides have a higher rate of yield is 5.064%, Germanium- rich of Flammulina velutipes polysaccharides rate higher than the yield of normal Flammulina velutipes polysaccharides, 7.741%. By contrast demonstrated, determination of antioxidant extracted water when mycelium is better than ethanol, germanium-rich group of SOD activity and inhibition of hydroxyl free radical 41.116, respectively U/mL and 34.674 U/mL, white group of SOD activity and inhibition of hydroxyl radical 32.49 U/mL and 29.006 U/mL, respectively. Measuring serum enriched germanium can be seen in mice Group's content of SOD and content of MDA of 90.27 respectively U/mL and 9.381nmoL/mL blank group content of SOD and content of MDA of 84.74 U/mL and 12.201 nmoL/mL, respectively. Germanium-rich antioxidant, anti-aging capabilities of Flammulina velutipes is significantly higher than normal Flammulina velutipes.

Key words: Flammulina velutipes, trace element; germanium (Ge); antioxidant; anti-aging