基于微流控芯片的肽库筛选_生物技术.rar

摘 要:芯片实验室（Lab on a chip）技术已发展成为当今世界上最前沿的科技领域之一，其以微流控芯片为核心，因高效快速、通量集成、节能低耗等特点在化学、生物学等领域的应用中彰显出独特的优势。多肽作为重要的内源性物质，在生物体内发挥重要的生理功能，多肽类药物、疫苗以及诊断试剂已成为生物医药领域的研究热点。本论文将多肽筛选与微流控芯片技术相结合，充分发挥微流控芯片的优势，构建了微流控芯片的多肽筛选识别新系统，开展了基于微流控芯片的多肽筛选与检测研究。主要研究结果如下：

　　　选取生理活性多肽特定位点的氨基酸，进行组合化学肽库合成，采用混合-裂分法构建了“一珠一物”多肽文库，库容量为105；将靶标蛋白与文库中的多肽微珠相互作用，采用生物素-链霉亲和素桥连及磁珠捕获的方法对多肽微珠进行特异性筛选；相对于传统的基于光学方法检测的多肽文库筛选，该方法具有高效、简便特点，又由于其无假阴性，可将大容量的肽库进行简并；初步将该筛选体系应用与于微流控系统，构建自动化、高效率、高通量的组合化学多肽筛选平台。

关键词：微流控芯片  多肽  筛选

Abstract:Lab on a chip technology has developed into the world's most cutting-edge science and technology, one of the microfluidic chips as the core, fast and efficient the flux integrated, low energy features in the field of chemistry, biology and other applicationshighlights the unique advantages. Polypeptide as an important endogenous substance, in vivo play an important physiological function of peptide drugs, vaccines and diagnostic reagents has become a hot research field of biomedicine. This article peptide screening and microfluidic chip technology combined. Give full play to the advantages of the microfluidic chip, built microfluidic chip peptide screening to identify new system, carried out the microfluidic chip-based peptide screening and testing research. The main findings are as follows:

　　　Select specific sites of the physiologically active polypeptide of the amino acid synthetic peptide library for combinatorial chemistry, mixed - splitting method to build a  " one bead one compound." polypeptide library, a storage capacity of 105; The interaction between the target protein and the library of polypeptides of microbeads, using biotin - streptavidin, and prime unbridged and bead capture method, the polypeptide microbeads specificity screening; Compared with the traditional optical detection-based peptide library screening, the method is an efficient, simple features, but also because of its false negative, the large-capacity peptide library can be simplified; The initial application of this screening system with combinatorial chemistry polypeptide in microfluidic systems, building automation, high efficiency, high-throughput screening platform.                    

Key Words：Microfluidic chips  Peptide  Filter