Hg2+及Hg2+、Cu2+复合离子对蚕豆根尖细胞微核的影响.doc

摘要：本实验以蚕豆根尖为供试材料，采用实验分析与统计分析的方法，用不同浓度的Hg2+离子（Hg2+离子浓度为5mg/L～100mg/L）及Hg2+、Cu2+复合离子（Hg2+离子浓度为5mg/L～100mg/L，每个浓度分别加入50mg/L的Cu2+离子）污染蚕豆根尖，探讨Hg2+离子及Hg2+、Cu2+复合离子对蚕豆根尖细胞的微核效应。此技术可用于对环境的监测。实验以蒸馏水处理为空白对照，结果表明不同浓度的Hg2+离子及Hg2+、Cu2+复合离子可使蚕豆根尖细胞微核率明显不同，说明Hg2+、Cu2+对豆根尖具有明显的毒性效应——诱发突变(微核) 。并且，随着Hg2+离子浓度的上升，微核率也随之上升(当Hg2+离子溶液浓度在5mg/L～50mg/L之间，随着浓度的上升，微核率随之上升；当Hg2+、Cu2+复合离子溶液浓度在5mg/L～20mg/L之间，随着浓度的上升，微核率也上升），但当达到一定浓度之后（Hg2+离子为50mg/L，微核率为48.51‰；Hg2+、Cu2+复合离子为20mg/L，微核率为19.47‰），微核率开始下降。实验中最低微核率出现在复合离子溶液最高浓度中（100 mg/L）。

关键词：Cu2+； Hg2+； 蚕豆； 根尖细胞； 微核率； 拮抗作用； 细胞遗传学毒理

Abstract:This project chooses vicia faba tip as material, by using the methods of experimental analysis and statistical analysis, with different concentrations of Hg2+ (Hg2+ concentration from 5mg / L to100 mg / L)and the combination f Hg2+ and Cu2+ (Hg2+ concentration from 5mg / L to 100 mg / L ,each concentration were added to 50mg/L of Cu2+) pollute vicia faba root tip to explore the micronucleus effect on vicia faba root tip induced by Hg2+ only and the combination of Hg2+ and Cu2+.This technology can be used to monitor the environment.This experiment use distilled water as a blank control test,whose results showed that different concentrations of Hg2+ only and the combination of Hg2+ and Cu2+ can make micronucles rate of vicia faba root tip cells significantly different ,this phenomenon shows that Hg2+ and Cu2+ have cytogenetic toxic effect on the root tip cells of vicia faba root tip cells (micronucles) . And with the rise of Hg2+ concentration, the micronucleus rate rises too (when Hg2+ only concentration between 5mg/L and 50mg/L, with the rise of the concentration, the micronucleus rate rises; when the combination solution of Hg2+ and Cu2+ between 5mg / L and 20 mg / L, with the concentration rise ,the micronucleus rate rises too), but when the concentration reaches a certain level (Hg2+ of 50 mg/L,the micronucleus rate is 48.51‰;the combination of Hg2+ and Cu2+ of 20mg/L,the micronucleus rate is 19.47‰),the rate of micronucleus start to decline. Minimum micronucleus rate in the experiment appear in the highest concentration of the combination solution(100mg/L). 

Keywords: Cu2+; Hg2+; vicia faba; root cells; micronucleus rate; antagonism; toxicology genetics