茯苓中5种有机磷农药残留量的同时测定.doc

摘要:建立了用配有氮磷检测器( NPD) 的气相色谱仪测定茯苓中敌敌畏、甲胺磷、乐果、对硫磷、辛硫磷5种农药残留量同时测定的方法。实验中采用超声波辅助混合溶剂提取，弗罗里硅土柱净化，浓缩，丙酮定容，用气相色谱-NPD检测器测定，峰面积外标法定量。结果显示有机磷标准品混标在0.005-1.0ug/mL的浓度范围内线性良好，在0.5、0.1、0.05ug/mL三个添加水平回收率分别为90.5%～105.3%,91.7%～01.7和84.1%～91.0%，相对标准偏差为1.47%～7.31%,3.11%～8.17%和4.68%～6.37%。实验结果表明，该方法各项指标均满足国内有机磷农药残留的检测要求。

关键词：有机磷农药残留；茯苓；气相色谱

ABSTRACT:A combined method for the determination of Dichlorvos,Methamidophos，Dimethoate， Parathion，Phoxim etc 5 Pesticide Residues. During the experiment,we use Ultrasonic assist Mixed solvent to Extract and Florisil column to Purification and Concentrate, Acetone to Constant volume, and also use gas chromatography with nitrogen-phosphorus detector  to Determinate combine with External standard method to quantitative. The results show that Organic phosphorus standard as Mixed standard its Concentration is good linearity around from 0.005 to 1.0ug/mL , and the sample that Concentration were 0.5,0.1 and 0.05 ug/mL that add The level of recovery were 90.5%-105.3%,91.7%～101.7 %and 84.1%～91.0%,the relative standard deviation were 1.47%～7.31%,3.11%～8.17% and 4.68%～6.37%.The experiment results show that the Indicators of this method are meet the national testing requirements on Organophosphorus pesticide residues.

Keywords: organophosphorus pesticide residues ； poria cocos ； gas chromatography