摘要:为优化高良姜黄酮的提取工艺,通过单因素试验考察了提取温度、提取时间、乙醇体积分数和液料比对高良姜黄酮得率的影响,在单因素试验的基础上,采用Box-Behnken设计,建立并分析了各因素与黄酮得率关系的数学模型。结果表明:高良姜黄酮提取的最佳工艺条件为提取温度85.9℃,提取时间3 h,乙醇体积63.56%,液料比25 mL/g,在此条件下高良姜黄酮得率的最大理论值为63.67 mg/g,该回归模型具有较好的预测性能,可用于指导生产实践。
关键词: 高良姜;黄酮;提取;响应面法
Abstract:Extraction technology of flavonoids from Alpinia officinarum Hance was optimized. Effects of extraction temperature, extraction time, ethanol concentration and liquid-to-solid ratio on the yield of flavonoids from Alpinia officinarum Hance were investigated through the single factor experiments. Based on the single factor experiments, technology parameters were optimized by Box-Behnken statistical design. The results show that when the extraction parameters were controlled at extraction temperature of 85.9℃ the optimum extracting conditions of Flavonoids from Alpinia officinarum Hance was 2 at , extraction time of 3 h, ethanol concentration of 63.56%, liquid-to-solid ratio 25 mL/g, the yield of Alpinia officinarum flavonoids could be up to 63.67 mg/g. The regression model has better prediction performance, can be used to guide the practical production
Keywords: Alpinia officinarum Hance; Flavonoids; Extraction; Response surface methodology