超声结合酶法提取杏鲍菇多糖及抗氧化活性的研究_食品科学与工程.rar

摘要:本实验采用超声结合酶法研究了杏鲍菇多糖提取的工艺条件，用苯酚一硫酸法测定多糖含量，用Sevag法脱蛋白对杏鲍菇多糖进行纯化，并对其抗氧化活性进行探讨。实验结果表明：复合酶最佳配比为纤维素酶1.0%，酸性蛋白酶1.0%，糖化酶0.6%，杏鲍菇多糖提取率为14.21%；最佳提取工艺条件为料液比1:25，超声时间15min，超声功率125W，杏鲍菇多糖提取率为14.56%。杏鲍菇多糖经脱蛋白后，多糖纯度提高了37.08%。杏鲍菇多糖对OH·、O2-·均有较强的清除能力，其50%清除率对应的杏鲍菇多糖浓度（IC50）分别为2.84mg/mL、2.64mg/mL。杏鲍菇多糖对DPPH也具有一定的清除作用，清除率可达60%以上。

关键词 杏鲍菇；多糖；复合酶；超声；抗氧化活性

Abstract:In our experiment, we took complex enzymatic extraction method and ultrasound combined with enzymatic to optimize the extracting conditions of yam polysaccharide, using the method of phenol-sulfuric to determine polysaccharide content and using the method of Sevag to remove protein from Pleurotus Eryngii polysaccharide and doing the research of antioxidant activity. The experimental results showed that: the optimal conditions for the extraction of complex enzymatic were cellulose enzyme was 1%, that saccharifying enzyme was 0.6%, and that acid protease was 1%,Pleurotus Eryngii polysaccharide extraction rate under these conditions was 14.21%; the optimal conditions for ultrasound combined with enzymatic extraction were that solid-to-liquid ratio of 1:25, ultrasonic time of 20min, and ultrasonic power of 150W,Pleurotus Eryngii polysaccharide extraction rate under these conditions was 14.56%.Pleurotus Eryngii polysaccharide increased the purity of the 37.08% through removing protein.Pleurotus Eryngii polysaccharide had a strong scavenging capacity on OH·、O2-·and their 50% clearance rate (IC50) are 2.84mg/mL、2.64mg/mL. Eryngii polysaccharide also had a strong scavenging capacity on DPPH,and its 50% clearance rate (IC50) catch 60%.

Keywords  Pleurotus Eryngii     Polysaccharide   Complex Enzymatic   Ultrasound Antioxidant activity