摘要:研究低聚异麦芽糖对小鼠胃肠道菌群及润肠通便功能的影响。
方法：以BALB/C小鼠为实验对象，调节菌群实验在连续给予低聚异麦芽糖14天后，测定小鼠粪便中肠杆菌、乳杆菌、双歧杆菌、肠球菌的含量；润肠通便实验在连续给予低聚异麦芽糖21天后，以复方地芬诺酯建立小鼠便秘模型，测定给予低聚异麦芽糖后小鼠排首黑便时间、6小时内黑便重量及小肠推进率。
结果：服用剂量为0.5mg/mL.bw低聚异麦芽糖的小鼠在受食前后，体重无明显变化；调节菌群实验，与空白对照组相比，连续给予低聚异麦芽糖14天后，乳杆菌增加4%（P＜0.05），双歧杆菌增加8%（P<0.01）；将各组灌服前后的结果做自身比较，空白对照组肠球菌增加14%（P<0.05），乳杆菌增加9%（P<0.01），低聚异麦芽糖组乳杆菌增加12%（P<0.01），双歧杆菌增加15%（P<0.01）。润肠通便实验，与模型对照组比较，连续给予低聚果糖21天后，首粒排黑便时间缩短了86% (P<0.01)，排黑便总质量增加77%（P<0.05），小肠推进率提高97%（P<0.01）。
结论：1.0g/kg.bw剂量的低聚异麦芽糖具有改善小鼠胃肠道菌群及润肠通便作用。
关键词：低聚异麦芽糖 胃肠道菌群 润肠通便
Abstract:To study the effect of Isomaltooligosaccharide on promoting multiplication of intestinal microbial flora and laxative function.
Methods: The BALB / C mice excrements CFUs of Bifidobacterium, Lactobacillus, Enterobacter and Enterococci were examined before and after to give oligosaccharides for 14 days. After continuous administration of oligosaccharides for 21 days, the first balck excrement, the weight of excrement within 6 hours and small intestine advance rate were determined on constipation mice.
Results: Compare with control group, with the administration of Isomaltooligosaccharide for 14 days, the intestine CFUs of Lactobacillus, Bifidobacterium were significantly increased 4%（P<0.01）and 8%（P<0.01）. Compare with the CFUs before to give test materials, the CFUs of Enterococci and Lactobacillus of control group were extremely raised 14%（P<0.05）and 9%（P<0.01）. The CFUs of Lactobacillus and Bifidobacterium of Isomaltooligosaccharide group were distinctly increased 12%（P<0.01）and 15%（P<0.01）respectively. With the administration for 21 days, the first black excrement obviously shortened 86%(P<0.01), the weight of excrement within 6 hours and small intestine advance rate extremely enhanced 77 %（P<0.05）and97%（P<0.01）.
Conclusion: The Isomaltooligosaccharide of 1.0g/kg.bw dose have regulative effects on intestinal microbial flora and prevent mice from constipation.
Key Words：Isomaltooligosaccharide Gastrointestinal tract flora Laxative
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