紫茎泽兰中黄酮提取工艺研究_设施农业科学与工程.doc

摘  要

　　　本试验以紫茎泽兰为材料，用不同浓度的乙醇作为提取剂，采用不同乙醇浓度梯度、不同浸泡时间对紫茎泽兰提取不同器官（根、茎、叶）中的总黄酮。结果表明：在紫茎泽兰总黄酮提取中，乙醇为75%，浸泡时间为28h，叶中总黄酮含量最高，为4.47%，茎中总黄酮含量次之，根中总黄酮含量最少。本试验以芦丁为对照品，Na2NO2-Al(NO3) 3-NaOH 体系为显色剂，利用分光光度法测定了紫茎泽兰中的总黄酮含量，平均回收率为97.2%,RSD=0.38 (n=5)。本法操作简便，可行，重现性好。

关键词：紫茎泽兰；总黄酮；提取工艺；研究

ABSTRACT

　　　In this experiment, the proliferation medium is Eupatorium adenophorum sprang.The extraction process of falconoid from Eupatorium adenophorum sprang was studied. The quantity proportion of falconoid extracted from Eupatorium adenophorum sprang was researched. The result indicated that under the extraction condition was as follow: ethanol concentration was 75%,the ration ,the extracting time was 28 hours, the extraction technological conditions of total flavonoids from sinesis leaves were optimized through orthogonal design method, and the extraction of leaf rate was 4.47%.The extraction of stem was second, The extraction of root was lowest .The average recovery of this method was 97.2%,and the relative standard deviation was 0.38（n=5）.The sample dilutes directly for determination. This analysis method develop was simple, fast and accurate.

Key words: Eupatorium adenophorum; Tota falconoid; Extraction technology; Study