石榴皮丙酮提取物的抗氧化性研究.doc

摘要:蒙自石榴占全国石榴总量的三分之一，不管在种植面积还是石榴品质上都是处于全国领先的位置，但是蒙自石榴的深加工工艺还未成熟。目前，国内外已经对许多植物的果皮进行了多酚类物质的提取和应用研究，如：葡萄皮、香蕉皮等的研究，而对石榴皮作为一种重要的多酚资源尚未见研究利用的报道。本文以采摘后的蒙自甜石榴皮为原料，用丙酮作为提取剂采用索氏回流提取，对其石榴皮丙酮提取物，通过FRAP实验进行抗氧化性研究，从而找出最佳提取条件为：6号筛（100目）、提取前浸泡1.5h、溶剂浓度为85%丙酮、提取时间30min、料液比2:30。在此条件下：索氏回流提取效果最好，硫酸亚铁含量为81.971umol/L。同时：多酚、黄酮含量与所做抗氧化性实验所得的硫酸亚铁含量正相关，即在上述最佳条件下，总多酚含量为11.257mg/g，总黄酮含量为1.627mg/g。通过对POV抗氧化比较石榴皮丙酮提取物与其他抗氧化剂（如VC、VE等）的对猪油抗氧化性差异；可知总体来说维生素E的抗氧化性显著比维生素C和石榴皮丙酮提取物抗氧化性高的多。然而从本实验可看出石榴皮丙酮提物具有一定抗氧化活性。

关键词：石榴皮；丙酮；抗氧化性

ABSTRACT:Mengzi accounting pomegranate pomegranate one-third of the total, regardless of the quality of the planting area or pomegranate are leading position in the country, but the deep processing of pomegranate Mengzi not yet mature. At present, the domestic fruit of many plants have been conducted on the extraction of polyphenols and applied research, such as: grape skins, banana peels and other research, while the polyphenol granatum as an important research and use of resources has not been reported. In this paper, Mengzi after picking sweet pomegranate rind as raw materials, with acetone as the extraction solvent extraction by Soxhlet reflux, acetone extract of pomegranate peel them, through the antioxidant activity of FRAP experiments to find out the optimum extraction conditions were: 6 sieve (100 mesh), extracted before the soaking 1.5h, 85% acetone solvent concentration, extraction time of 30min, solid to liquid ratio of 2:30. Under these conditions: the best Soxhlet reflux extraction, ferrous sulfate content 81.971umol/L. At the same time: polyphenols, flavonoids and antioxidant activity made from ferrous sulfate experiment positively correlated, that is, the optimum conditions, the total polyphenol content was 11.257mg/g, total flavonoids content was 1.627mg/g. POV oxidation by acetone extracts of pomegranate peel compared with other antioxidants (such as VC, VE, etc.) between antioxidant activity of lard; we can see the whole antioxidant vitamin E, vitamin C and significantly higher than granatum Acetone Extract Antioxidative higher. However, this experiment can be seen from the acetone extract of pomegranate peel has some antioxidant activity.

Key words: Pomegranate peel;Acetone;Saxhlet reflux extraction